Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories.

ABSTRACT: The goal of this work was to improve the lycopene storage capacity of the E. coli membrane by engineering both morphological and biosynthetic aspects. First, Almgs, a protein from Acholeplasma laidlawii that is involved in membrane bending is overexpressed to expand the storage space for lycopene, which resulted in a 12% increase of specific lycopene production. Second, several genes related to the membrane-synthesis pathway in E. coli, including plsb, plsc, and dgka, were also overexpressed, which led to a further 13% increase. In addition, membrane separation and component analysis confirmed that the increased amount of lycopene was mainly accumulated within the cell membranes. Finally, by integrating both aforementioned modification strategies,

SUBMITTER: Wu T 

PROVIDER: S-EPMC5970105 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature