Identification of recombination events in outbred species with next-generation sequencing data.

ABSTRACT:

Background

Meiotic recombination events include crossovers and non-crossovers or gene conversions. Although the rate of crossovers is often used for genetic mapping, the gene conversion events are not well studied especially in outbred species, which could produce distorted markers and thus affect the precision of genetic maps.

Results

We proposed a strategy for identifying gene conversion events in Populus with the next-generation sequencing (NGS) data from the two parents and their progeny in an F₁ hybrid population. The strategy first involved phasing the heterozygous SNPs of the parents to obtain the parental haplotype blocks by NGS analytical tools, permitting to identify the parental gene conversion events with progeny genotypes. By incorporating available g

SUBMITTER: Tao S 

PROVIDER: S-EPMC5970487 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature