Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep.

ABSTRACT:

Background

The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead to negative consequences. Especially, a very recent study found that gene editing can introduce hundreds of unintended mutations into the genome, and have attracted wide attention.

Results

To address the off-target concerns, urgent characterization of the CRISPR/Cas9-mediated off-target mutagenesis is highly anticipated. Here we took advantage of our previously generated gene-edited sheep and performed family trio-based whole genome sequencing which is capable of discriminating variants in

SUBMITTER: Wang X 

PROVIDER: S-EPMC5970491 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature