Reversing the direction of galvanotaxis with controlled increases in boundary layer viscosity.

ABSTRACT: Weak external electric fields (EFs) polarize cellular structure and direct most migrating cells (galvanotaxis) toward the cathode, making it a useful tool during tissue engineering and for healing epidermal wounds. However, the biophysical mechanisms for sensing weak EFs remain elusive. We have reinvestigated the mechanism of cathode-directed water flow (electro-osmosis) in the boundary layer of cells, by reducing it with neutral, viscous polymers. We report that increasing viscosity with low molecular weight polymers decreases cathodal migration and promotes anodal migration in a concentration dependent manner. In contrast, increased viscosity with high molecular weight polymers does not affect directionality. We explain the contradictory results in terms of porosity and hydraulic permeab

SUBMITTER: Kobylkevich BM 

PROVIDER: S-EPMC5970543 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature