Transcriptomics of dorso-ventral axis determination in Xenopus tropicalis.

ABSTRACT: Amphibian embryos provide a powerful system to study early cell fate determination because their eggs are externally fertilised, large, and easy to manipulate. Ultraviolet (UV) or lithium chloride (LiCl) treatment are classic embryonic manipulations frequently used to perturb specification of the dorso-ventral (DV) axis by affecting the stability of the maternal Wnt mediator β-catenin. Such treatments result in the formation of so-called ventralised or dorsalised embryos. Although these phenotypes have been well described with respect to their morphology and some aspects of gene expression, their whole transcriptomes have never been systematically characterised and compared. Here we show that at the early gastrula stage UV-treated embryos are transcriptionally more closely related to untre

SUBMITTER: Monteiro RS 

PROVIDER: S-EPMC5971218 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature