T_m-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum.

ABSTRACT: To develop a T_m-shift method for detection of dog-derived Ancylostoma ceylanicum and A. caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the T_m-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between A. ceylanicum and A. caninum. The coefficient of variation in their T_m values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for A. ceyl

SUBMITTER: Fu Y 

PROVIDER: S-EPMC5971263 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature