Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.

ABSTRACT:

Background

Enhancers and promoters are cis-acting regulatory elements associated with lineage-specific gene expression. Previous studies showed that different categories of active regulatory elements are in regions of open chromatin, and each category is associated with a specific subset of post-translationally marked histones. These regulatory elements are systematically activated and repressed to promote commitment of hematopoietic stem cells along separate differentiation paths, including the closely related erythrocyte (ERY) and megakaryocyte (MK) lineages. However, the order in which these decisions are made remains unclear.

Results

To characterize the order of cell fate decisions during hematopoiesis, we collected primary cells from mouse bone marrow and isolated 10 he

SUBMITTER: Heuston EF 

PROVIDER: S-EPMC5971425 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature