The yeast protein Gdt1p transports Mn²⁺ ions and thereby regulates manganese homeostasis in the Golgi.

ABSTRACT: The uncharacterized protein family 0016 (UPF0016) is a family of secondary ion transporters implicated in calcium homeostasis and some diseases. More precisely, genetic variants of the human UPF0016 ortholog transmembrane protein 165 (TMEM165) have been linked to congenital disorders of glycosylation (CDG). The Saccharomyces cerevisiae ortholog Gdt1p has been shown to be involved in calcium homeostasis and protein glycosylation. Moreover, plant and bacterial UPF0016 members appear to have putative roles in Mn²⁺ homeostasis. Here, we produced the yeast UPF0016 member Gdt1p in the bacterial host Lactococcus lactis Using Mn²⁺-induced quenching of Fura-2-emitted fluorescence, we observed that Gdt1p mediates Mn²⁺ influx, in addition to its previous

SUBMITTER: Thines L 

PROVIDER: S-EPMC5971465 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature