Endoribonuclease YbeY Is Linked to Proper Cellular Morphology and Virulence in Brucella abortus.

ABSTRACT: The YbeY endoribonuclease is one of the best-conserved proteins across the kingdoms of life. In the present study, we demonstrated that YbeY in Brucella abortus is linked to a variety of important activities, including proper cellular morphology, mRNA transcript levels, and virulence. Deletion of ybeY in B. abortus led to a small-colony phenotype when the bacteria were grown on agar medium, as well as to significant aberrations in the morphology of the bacterial cell as evidenced by electron microscopy. Additionally, compared to the parental strain, the ΔybeY strain was significantly attenuated in both macrophage and mouse models of infection. The ΔybeY strain also showed increased sensitivities to several in vitro-applied stressors, including bile

SUBMITTER: Budnick JA 

PROVIDER: S-EPMC5971472 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature