High-Speed Single-Molecule Tracking of CXCL13 in the B-Follicle.

ABSTRACT: Soluble factors are an essential means of communication between cells and their environment. However, many molecules readily interact with extracellular matrix components, giving rise to multiple modes of diffusion. The molecular quantification of diffusion in situ is thus a challenging imaging frontier, requiring very high spatial and temporal resolution. Overcoming this methodological barrier is key to understanding the precise spatial patterning of the extracellular factors that regulate immune function. To address this, we have developed a high-speed light microscopy system capable of millisecond sampling in ex vivo tissue samples and submillisecond sampling in controlled in vitro samples to characterize molecular diffusion in a range of complex microenvironments.

SUBMITTER: Miller H 

PROVIDER: S-EPMC5972203 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature