Glucose-Dependent Promoters for Dynamic Regulation of Metabolic Pathways.

ABSTRACT: For an industrial fermentation process, it can be advantageous to decouple cell growth from product formation. This decoupling would allow for the rapid accumulation of biomass without inhibition from product formation, after which the fermentation can be switched to a mode where cells would grow minimally and primarily act as catalysts to convert substrate into desired product. The switch in fermentation mode should preferably be accomplished without the addition of expensive inducers. A common cell factory Saccharomyces cerevisiae is a Crabtree-positive yeast and is typically fermented at industrial scale under glucose-limited conditions to avoid the formation of ethanol. In this work, we aimed to identify and characterize promoters that depend on glucose concentration for use as

SUBMITTER: Maury J 

PROVIDER: S-EPMC5972318 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature