Inflammation leads to distinct populations of extracellular vesicles from microglia.

ABSTRACT:

Background

Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS). Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication. However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain. Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content.

Methods

We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell lin

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5972400 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature