Clonal CD4⁺ T cells in the HIV-1 latent reservoir display a distinct gene profile upon reactivation.

ABSTRACT: Despite suppressive combination antiretroviral therapy (ART), latent HIV-1 proviruses persist in patients. This latent reservoir is established within 48-72 h after infection, has a long half-life^1,2, enables viral rebound when ART is interrupted, and is the major barrier to a cure for HIV-1 ³ . Latent cells are exceedingly rare in blood (∼1 per 1 × 10⁶ CD4⁺ T cells) and are typically enumerated by indirect means, such as viral outgrowth assays^4,5. We report a new strategy to purify and characterize single reactivated latent cells from HIV-1-infected individuals on suppressive ART. Surface expression of viral envelope protein was used to enrich reactivated latent T cells producing HIV RNA, and single-cell analysis was performed to iden

SUBMITTER: Cohn LB 

PROVIDER: S-EPMC5972543 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature