Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach.

ABSTRACT: Antibodies are routinely used to study the activity of transcription factors, using various in vitro and in vivo approaches such as electrophoretic mobility shift assay, enzyme-linked immunosorbent assay, genome-wide method analysis coupled with next generation sequencing, or mass spectrometry. More recently, a new application for antibodies has emerged as crystallisation scaffolds for difficult to crystallise proteins, such as transcription factors. Only in a few rare cases, antibodies have been used to modulate the activity of transcription factors, and there is a real gap in our knowledge on how to efficiently design antibodies to interfere with transcription. The molecular function of transcription factors is underpinned by complex networks of protein-protein interaction and in theory,

SUBMITTER: Fontaine FR 

PROVIDER: S-EPMC5972640 | biostudies-literature | 2018 May/Jun

REPOSITORIES: biostudies-literature