Cell Expansion During Directed Differentiation of Stem Cells Toward the Hepatic Lineage.

ABSTRACT: The differentiation of human pluripotent stem cells toward the hepatocyte lineage can potentially provide an unlimited source of functional hepatocytes for transplantation and extracorporeal bioartificial liver applications. It is anticipated that the quantities of cells needed for these applications will be in the order of 10⁹-10¹⁰ cells, because of the size of the liver. An ideal differentiation protocol would be to enable directed differentiation to the hepatocyte lineage with simultaneous cell expansion. We introduced a cell expansion stage after the commitment of human embryonic stem cells to the endodermal lineage, to allow for at least an eightfold increase in cell number, with continuation of cell maturation toward the hepatocyte lineage. The progressive chang

SUBMITTER: Raju R 

PROVIDER: S-EPMC5972777 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature