Functional Analysis of the Dengue Virus Genome Using an Insertional Mutagenesis Screen.

ABSTRACT: In the last few decades, dengue virus, an arbovirus, has spread to over 120 countries. Although a vaccine has been approved in some countries, limitations on its effectiveness and a lack of effective antiviral treatments reinforce the need for additional research. The functions of several viral nonstructural proteins are essentially unknown. To better understand the functions of these proteins and thus dengue virus pathogenesis, we embarked on a genomewide transposon mutagenesis screen with next-generation sequencing to determine sites in the viral genome that tolerate 15-nucleotide insertions. Using this approach, we generated support for several published predicted transmembrane and enzymatic domains. Next, we created 7 mutants containing the 15-nucleotide insertion from the original sel

SUBMITTER: Perry JW 

PROVIDER: S-EPMC5972875 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature