Isolation and characterization of Ulva prolifera actin1 gene and function verification of the 5' flanking region as a strong promoter.

ABSTRACT: Ulva prolifera is a green macroalgae with an extremely high growth rate that can accumulate biomass with considerable protein content. To set up an available seaweed expression system, a prior step is to isolate endogenous and strong constitutive promoters. For this reason, the full-length genomic actin1 gene from U. prolifera (Upactin1) was cloned and its 5' flanking sequence was obtained by genome walking. The Upactin1 open reading frame consisted of 1134 nucleotides encoding 377 amino acid residues. Besides 4 exons and 3 introns in the coding region, an extra leader intron was identified in the 5' untranslated region. According to quantitative GUS assays based on transient expression, the promoter activity of the Upactin1 5' flanking region was found to be several times higher than that

SUBMITTER: Wu C 

PROVIDER: S-EPMC5972938 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature