Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome.

ABSTRACT:

Background

A single-tube one-step real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of chikungunya virus (CHIKV) targeting the conserved 6K-E1 target region was developed. The assay was validated with sera collected from a CHIKV outbreak in Senegal in 2015.

Methodology/principal findings

A novel design approach by combining Principal Component Analysis and phylogenetic analysis of 110 available CHIKV sequences and the LAMP oligonucleotide design software LAVA was used. The assay was evaluated with an External Quality Assessment panel from the European Network for Diagnostics of "Imported" Viral Diseases and was shown to be sensitive and specific and did not cross-detect other arboviruses. The limit of detection as dete

SUBMITTER: Lopez-Jimena B 

PROVIDER: S-EPMC5973553 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature