Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype.

ABSTRACT:

Background

4 one-step, real-time, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed for the detection of dengue virus (DENV) serotypes by considering 2,056 full genome DENV sequences. DENV1 and DENV2 RT-LAMP assays were validated with 31 blood and 11 serum samples from Tanzania, Senegal, Sudan and Mauritania. DENV3 and DENV4 RT-LAMP assays were validated with 25 serum samples from Cambodia.

Methodology/principal findings

4 final reaction primer mixes were obtained by using a combination of Principal Component Analysis of the full DENV genome sequences, and LAMP primer design based on sequence alignments using the LAVA software. These mixes contained 14 (DENV1), 12 (DENV2), 8 (DENV3) and 3 (DENV4) LAMP primer sets. The assays were ev

SUBMITTER: Lopez-Jimena B 

PROVIDER: S-EPMC5973574 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature