Distinct chromatin functional states correlate with HIV latency reactivation in infected primary CD4⁺ T cells.

ABSTRACT: Human immunodeficiency virus (HIV) infection is currently incurable, due to the persistence of latently infected cells. The 'shock and kill' approach to a cure proposes to eliminate this reservoir via transcriptional activation of latent proviruses, enabling direct or indirect killing of infected cells. Currently available latency-reversing agents (LRAs) have however proven ineffective. To understand why, we used a novel HIV reporter strain in primary CD4⁺ T cells and determined which latently infected cells are reactivatable by current candidate LRAs. Remarkably, none of these agents reactivated more than 5% of cells carrying a latent provirus. Sequencing analysis of reactivatable vs. non-reactivatable populations revealed that the integration sites were distinguishable in term

SUBMITTER: Battivelli E 

PROVIDER: S-EPMC5973828 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature