Platinum sensitivity and DNA repair in a recently established panel of patient-derived ovarian carcinoma xenografts.

ABSTRACT: A xenobank of patient-derived (PDX) ovarian tumor samples has been established consisting of tumors with different sensitivity to cisplatin (DDP), from very responsive to resistant. As the DNA repair pathway is an important driver in tumor response to DDP, we analyzed the mRNA expression of 20 genes involved in the nucleotide excision repair, fanconi anemia, homologous recombination, base excision repair, mismatch repair and translesion repair pathways and the methylation patterns of some of these genes. We also investigated the correlation with the response to platinum-based therapy. The mRNA levels of the selected genes were evaluated by Real Time-PCR (RT-PCR) with ad hoc validated primers and gene promoter methylation by pyrosequencing. All the DNA repair genes were variably expr

SUBMITTER: Guffanti F 

PROVIDER: S-EPMC5973859 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature