Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages.

ABSTRACT: High androgen level impairs endometrial receptivity in women experiences the recurrent miscarriage. The mechanism of androgen actions on endometrium is still uncertain. We hypothesized that androgen has a direct effect on the endometrium in women with recurrent miscarriage. In the present study, we assess the impact of androgen (A₂) at high concentration (10^-7 M) on Ishikawa cells compared with the physiological concentration of androgen (10^-9 M). To go into deeper analysis, we use global stable isotopes labeled profiling tactic using iTRAQ reagents, followed by 2D LC-MS/MS. We determine 175 non-redundant proteins, and 18 of these were quantified. The analysis of differentially expressed proteins (DEPs) identified 8 up-regulated proteins and 10 down-regulat

SUBMITTER: Rahman TU 

PROVIDER: S-EPMC5973874 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature