Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics.

ABSTRACT: Sample preparation for quantitative proteomics is a crucial step to ensure the repeatability and the accuracy of the results. However, there is no universal method compatible with the wide variety of protein extraction buffers currently used. We have recently demonstrated the compatibility of tube-gel with SDS-based buffers and its efficiency for label-free quantitative proteomics by comparing it to stacking gel and liquid digestion. Here, we investigated the compatibility of tube-gel with alternatives to SDS-based buffers allowing notably the extraction of proteins in various pH conditions. We also explored the use of photopolymerization to extend the number of possibilities, as it is compatible with a wide range of pH and is non-oxidative. To achieve this goal, we compared six extraction

SUBMITTER: Muller L 

PROVIDER: S-EPMC5974325 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature