Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory.

ABSTRACT: Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway performance. One strategy, however, may produce disparate results for different biosynthetic pathways. We use a spatially resolved kinetic model to explore this fundamental design choice in systems and synthetic biology. We predict that two example biosynthetic pathways have distinct optimal organization strategies that vary based on pathway-dependent and cell-extrinsic factors. Moreover, we demonstrate that the optimal design varies as a function of kinetic and biophysical properties, as well as culture conditions. Our results suggest that organ

SUBMITTER: Jakobson CM 

PROVIDER: S-EPMC5974357 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature