Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates.

ABSTRACT: Here, we report results on systematic analysis of DNA substrate preferences of three N6-adenine β-class DNA methyltransferases that are part of the type II restriction-modification systems. The studied enzymes were: M.EcoVIII, M.HindIII and M.LlaCI, which although found in phylogenetically distant bacteria (γ-proteobacteria and low-GC Gram-positive bacteria), recognize the same palindromic specific sequence 5'-AAGCTT-3' and catalyze formation of N6-methyladenine at the first A-residue. As expected overall the enzymes share the most analyzed features, but they show also some distinct differences in substrate recognition. Therefore DNA methylation reactions were carried out not only under standard, but also under relaxed conditions using DMSO or glycerol. We found that all of these enzymes p

SUBMITTER: Wons E 

PROVIDER: S-EPMC5974420 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature