Assessment of stem cell differentiation based on genome-wide expression profiles.

ABSTRACT: In recent years, protocols have been established to differentiate stem and precursor cells into more mature cell types. However, progress in this field has been hampered by difficulties to assess the differentiation status of stem cell-derived cells in an unbiased manner. Here, we present an analysis pipeline based on published data and methods to quantify the degree of differentiation and to identify transcriptional control factors explaining differences from the intended target cells or tissues. The pipeline requires RNA-Seq or gene array data of the stem cell starting population, derived 'mature' cells and primary target cells or tissue. It consists of a principal component analysis to represent global expression changes and to identify possible problems of the dataset that require spec

SUBMITTER: Godoy P 

PROVIDER: S-EPMC5974444 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature