Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

ABSTRACT: Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via en masse "party" mating can also be monitored en masse for growth to detect genetic interactions. By using site-specific recombination to fuse two DNA barcodes, each representing a specific gene deletion, BFG-GI enables multiplexed quantitative tracking of double mutants via next-generation sequencing. We applied BFG-GI to a matrix of D

SUBMITTER: Diaz-Mejia JJ 

PROVIDER: S-EPMC5974512 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature