Confirmation of Drought Tolerance of Ectopically Expressed AtABF3 Gene in Soybean.

ABSTRACT: Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from T₂ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment sh

SUBMITTER: Kim HJ 

PROVIDER: S-EPMC5974618 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature