Apolipoprotein A-1 mimetic peptide 4F promotes endothelial repairing and compromises reendothelialization impaired by oxidized HDL through SR-B1.

ABSTRACT: Disruption of endothelial monolayer integrity is the primary instigating factor for many cardiovascular diseases. High density lipoprotein (HDL) oxidized by heme enzyme myeloperoxidase (MPO) is dysfunctional in promoting endothelial repair. Apolipoprotein A-1 mimetic 4F with its pleiotropic benefits has been proven effective in many in vivo models. In this study we investigated whether 4F promotes endothelial repair and restores the impaired function of oxidized HDL (Cl/NO₂-HDL) in promoting re-endothelialization. We demonstrate that 4F and Cl/NO₂-HDL act on scavenger receptor type I (SR-B1) using human aorta endothelial cells (HAEC) and SR-B1 ^(-/-) mouse aortic endothelial cells. Wound healing, transwell migration, lamellipodia formation and single cell mi

SUBMITTER: He D 

PROVIDER: S-EPMC5975068 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature