Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.

ABSTRACT: Endothelial dysfunction is associated with decreased NO bioavailability and impaired activation of the NO receptor soluble guanylate cyclase (sGC) in the vasculature and in platelets. Red blood cells (RBCs) are known to produce NO under hypoxic and normoxic conditions; however evidence of expression and/or activity of sGC and downstream signaling pathway including phopshodiesterase (PDE)-5 and protein kinase G (PKG) in RBCs is still controversial. In the present study, we aimed to investigate whether RBCs carry a functional sGC signaling pathway and to address whether this pathway is compromised in coronary artery disease (CAD). Using two independent chromatographic procedures, we here demonstrate that human and murine RBCs carry a catalytically active α₁β₁-sGC (isofo

SUBMITTER: Cortese-Krott MM 

PROVIDER: S-EPMC5975213 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature