Identification of Cyclic Depsipeptides and Their Dedicated Synthetase from Hapsidospora irregularis.

ABSTRACT: Seven cyclic depsipeptides were isolated from Hapsidospora irregularis and structurally characterized as the calcium channel blocker leualacin and six new analogues based on the NMR and HRESIMS data. These new compounds were named leualacins B-G. The absolute configurations of the amino acids and 2-hydroxyisocaproic acids were determined by recording the optical rotation values. Biological studies showed that calcium influx elicited by leualacin F in primary human lobar bronchial epithelial cells involves the TRPA1 channel. Through genome sequencing and targeted gene disruption, a noniterative nonribosomal peptide synthetase was found to be involved in the biosynthesis of leualacin. A comparison of the structures of leualacin and its analogues indicated that the A₂ and A_4</

SUBMITTER: Zhang S 

PROVIDER: S-EPMC5975237 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature