Stability of gene expression by primary bronchial epithelial cells over increasing passage number.

ABSTRACT:

Background

An increasing number of studies using primary human bronchial epithelial cells (BECs) have reported intrinsic differences in the expression of several genes between cells from asthmatic and non-asthmatic donors. The stability of gene expression by primary BECs with increasing cell passage number has not been well characterized.

Methods

To determine if expression by primary BECs from asthmatic and non-asthmatic children of selected genes associated with airway remodeling, innate immune response, immunomodulatory factors, and markers of differentiated airway epithelium, are stable over increasing cell passage number, we studied gene expression patterns in passages 1, 2, 3, 4, and 5 BECs from asthmatic (n = 6) and healthy (n = 6) subjects that were differentiated at

SUBMITTER: Reeves SR 

PROVIDER: S-EPMC5975426 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature