Light sheet theta microscopy for rapid high-resolution imaging of large biological samples.

ABSTRACT:

Background

Advances in tissue clearing and molecular labeling methods are enabling unprecedented optical access to large intact biological systems. These developments fuel the need for high-speed microscopy approaches to image large samples quantitatively and at high resolution. While light sheet microscopy (LSM), with its high planar imaging speed and low photo-bleaching, can be effective, scaling up to larger imaging volumes has been hindered by the use of orthogonal light sheet illumination.

Results

To address this fundamental limitation, we have developed light sheet theta microscopy (LSTM), which uniformly illuminates samples from the same side as the detection objective, thereby eliminating limits on lateral dimensions without sacrificing the imaging resolution, depth,

SUBMITTER: Migliori B 

PROVIDER: S-EPMC5975440 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature