Next-generation sequencing library construction on a surface.

ABSTRACT: BACKGROUND:Next-generation sequencing (NGS) has revolutionized almost all fields of biology, agriculture and medicine, and is widely utilized to analyse genetic variation. Over the past decade, the NGS pipeline has been steadily improved, and the entire process is currently relatively straightforward. However, NGS instrumentation still requires upfront library preparation, which can be a laborious process, requiring significant hands-on time. Herein, we present a simple but robust approach to streamline library preparation by utilizing surface bound transposases to construct DNA libraries directly on a flowcell surface. RESULTS:The surface bound transposases directly fragment genomic DNA while simultaneously attaching the library molecules to the flowcell. We sequenced and analysed a Droso

SUBMITTER: Feng K 

PROVIDER: S-EPMC5975494 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature