Grifolic acid induces GH3 adenoma cell death by inhibiting ATP production through a GPR120-independent mechanism.

ABSTRACT:

Background

Grifolic acid is a derivative of grifolin, an antitumor natural compound, and it was reported as an agonist of free fatty acid receptor GPR120. Little is known about its antitumor effects and the involvement of GPR120.

Methods

GH3 cells, the rat anterior pituitary adenoma cells, were cultured and the cell death was measured by MTT assay and Annexin V/PI staining. The mitochondrial membrane potential (MMP) of GH3 cells was measured by JC-1 staining. Cellular ATP levels and the intracellular NAD/NADH ratio were measured. GPR120 expression in GH3 cells was observed by RT-PCR and Western Blot, and siRNA was used to inhibit GPR120 expression in GH3 cells.

Results

Grifolic acid dose- and time-dependently induced the necrosis of GH3 cells. Grifolic acid significan

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC5975534 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature