Development of a high-throughput flexible quantitative suspension array assay for IgG against multiple Plasmodium falciparum antigens.

ABSTRACT:

Background

Antibody responses to Plasmodium falciparum play a critical role in disease control. Finding reliable IgG biomarkers of protection is complicated by a parasite proteome of over 5000 proteins, some with polymorphisms. Studies of anti-malarial naturally acquired and vaccine immunity would benefit from a standard high-throughput immunoassay to measure multiple antibodies. A multiplex quantitative suspension assay to measure antigen-specific IgGs was developed and its precision (reproducibility and repeatability), dynamic range, limits of detection and quantification, and non-specific binding to different P. falciparum proteins tested. A set of 288 human plasma samples from a malaria-endemic region were analysed twice by two different operators. Another set of samples from 9

SUBMITTER: Ubillos I 

PROVIDER: S-EPMC5975539 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature