Diversity and signature of small RNA in different bodily fluids using next generation sequencing.

ABSTRACT:

Background

Small RNAs are critical components in regulating various cellular pathways. These molecules may be tissue-associated or circulating in bodily fluids and have been shown to associate with different tumors. Next generation sequencing (NGS) on small RNAs is a powerful tool for profiling and discovery of microRNAs (miRNAs).

Results

In this study, we isolated total RNA from various bodily fluids: blood, leukocytes, serum, plasma, saliva, cell-free saliva, urine and cell-free urine. Next, we used Illumina's NGS platform and intensive bioinformatics analysis to investigate the distribution and signature of small RNAs in the various fluids. Successful NGS was accomplished despite the variations in RNA concentrations among the different fluids. Among the fluids studied, bl

SUBMITTER: El-Mogy M 

PROVIDER: S-EPMC5975555 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature