Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation.

ABSTRACT: The three-dimensional organization of chromosomes within the nucleus and its dynamics during differentiation are largely unknown. To visualize this process in molecular detail, we generated high-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes. This reveals that a basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. This remodeling involves both individual transcription units and multigene regions and affects many genes that determine cellular identity. Often, genes that move away from the lamina are concomitantly act

SUBMITTER: Peric-Hupkes D 

PROVIDER: S-EPMC5975946 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature