Rapid and efficient genetic engineering of both wild type and axenic strains of Dictyostelium discoideum.

ABSTRACT: Dictyostelium has a mature technology for molecular-genetic manipulation based around transfection using several different selectable markers, marker re-cycling, homologous recombination and insertional mutagenesis, all supported by a well-annotated genome. However this technology is optimized for mutant, axenic cells that, unlike non-axenic wild type, can grow in liquid medium. There is a pressing need for methods to manipulate wild type cells and ones with defects in macropinocytosis, neither of which can grow in liquid media. Here we present a panel of molecular genetic techniques based on the selection of Dictyostelium transfectants by growth on bacteria rather than liquid media. As well as extending the range of strains that can be manipulated, these techniques are faster than convent

SUBMITTER: Paschke P 

PROVIDER: S-EPMC5976153 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature