Discovery of SNPs for individual identification by reduced representation sequencing of moose (Alces alces).

ABSTRACT: Monitoring of wild animal populations is challenging, yet reliable information about population processes is important for both management and conservation efforts. Access to molecular markers, such as SNPs, enables population monitoring through genotyping of various DNA sources. We have developed 96 high quality SNP markers for individual identification of moose (Alces alces), an economically and ecologically important top-herbivore in boreal regions. Reduced representation libraries constructed from 34 moose were high-throughput de novo sequenced, generating nearly 50 million read pairs. About 50 000 stacks of aligned reads containing one or more SNPs were discovered with the Stacks pipeline. Several quality criteria were applied on the candidate SNPs to find markers informative on the i

SUBMITTER: Blahed IM 

PROVIDER: S-EPMC5976195 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature