Kinetic and structural comparison of a protein's cotranslational folding and refolding pathways.

ABSTRACT: Precise protein folding is essential for the survival of all cells, and protein misfolding causes a number of diseases that lack effective therapies, yet the general principles governing protein folding in the cell remain poorly understood. In vivo, folding can begin cotranslationally and protein quality control at the ribosome is essential for cellular proteostasis. We directly characterize and compare the refolding and cotranslational folding trajectories of the protein HaloTag. We introduce new techniques for both measuring folding kinetics and detecting the conformations of partially folded intermediates during translation in real time. We find that, although translation does not affect the rate-limiting step of HaloTag folding, a key aggregation-prone intermediate observed during in v

SUBMITTER: Samelson AJ 

PROVIDER: S-EPMC5976279 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature