Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation in vertebrates.

ABSTRACT: As microtubule-organizing centers of animal cells, centrosomes guide the formation of the bipolar spindle that segregates chromosomes during mitosis. At mitosis onset, centrosomes maximize microtubule-organizing activity by rapidly expanding the pericentriolar material (PCM). This process is in part driven by the large PCM protein pericentrin (PCNT), as its level increases at the PCM and helps recruit additional PCM components. However, the mechanism underlying the timely centrosomal enrichment of PCNT remains unclear. Here, we show that PCNT is delivered co-translationally to centrosomes during early mitosis by cytoplasmic dynein, as evidenced by centrosomal enrichment of PCNT mRNA, its translation near centrosomes, and requirement of intact polysomes for PCNT mRNA localizat

SUBMITTER: Sepulveda G 

PROVIDER: S-EPMC5976437 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature