Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma.

ABSTRACT: Podocalyxin (PODXL) overexpression is associated with progression, metastasis, and poor outcomes in cancers. We recently produced the novel anti-PODXL monoclonal antibody (mAb) PcMab-47 (IgG₁, kappa). Herein, we engineered PcMab-47 into 47-mG_2a, a mouse IgG_2a-type mAb, to add antibody-dependent cellular cytotoxicity (ADCC). We further developed 47-mG_2a-f, a core fucose-deficient type of 47-mG_2a to augment its ADCC. Immunohistochemical analysis of oral cancer tissues using PcMab-47 and 47-mG_2a revealed that the latter stained oral squamous cell carcinoma (OSCC) cells in a cytoplasmic pattern at a much lower concentration. PcMab-47 and 47-mG_2a detected PODXL in 163/201 (81.1%) and in 197/201 (98.0%) OSCC samples, re

SUBMITTER: Itai S 

PROVIDER: S-EPMC5976479 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature