Label-Free, Flow-Imaging Methods for Determination of Cell Concentration and Viability.

ABSTRACT: PURPOSE:To investigate the potential of two flow imaging microscopy (FIM) techniques (Micro-Flow Imaging (MFI) and FlowCAM) to determine total cell concentration and cell viability. METHODS:B-lineage acute lymphoblastic leukemia (B-ALL) cells of 2 different donors were exposed to ambient conditions. Samples were taken at different days and measured with MFI, FlowCAM, hemocytometry and automated cell counting. Dead and live cells from a fresh B-ALL cell suspension were fractionated by flow cytometry in order to derive software filters based on morphological parameters of separate cell populations with MFI and FlowCAM. The filter sets were used to assess cell viability in the measured samples. RESULTS:All techniques gave fairly similar cell concentration values over the whole incubation peri

SUBMITTER: Sediq AS 

PROVIDER: S-EPMC5976703 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature