Targeted Transcriptional Profiling of Kidney Transplant Biopsies.

ABSTRACT:

Introduction

Studies are needed to assess the quality of transcriptome analysis in paired human tissue samples preserved by different methods and different gene amplification platforms to enable data comparisons across experimenters.

Methods

RNA was extracted from kidney biopsies, either submerged in RNA-stabilizing solution (RSS) or stored in formalin-fixed, paraffin-embedded (FFPE) blocks. RNA quality and integrity were compared. Gene expression of the common rejection module and other immune cell genes were quantified for both tissue preservation methods in the same sample using conventional quantitative polymerase chain reaction (QPCR) by 2 different commercial platforms, (fluidigm [FD]) or barcoded-oligos (nanostring [NS]).

Results

RNA quality was inferior in FFP

SUBMITTER: Sigdel TK 

PROVIDER: S-EPMC5976814 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature