Breast cancer cell obatoclax response characterization using passivated-electrode insulator-based dielectrophoresis.

ABSTRACT: Inherent electrical properties of cells can be beneficial to characterize different cell lines and their response to experimental drugs. This paper presents a novel method to characterize the response of breast cancer cells to drug stimuli through use of off-chip passivated-electrode insulator-based dielectrophoresis (OπDEP) and the application of AC electric fields. This work is the first to demonstrate the ability of OπDEP to differentiate between two closely related breast cancer cell lines, LCC1 and LCC9 while assessing their drug sensitivity to an experimental anti-cancer agent, Obatoclax. Although both cell lines are derivatives of estrogen-responsive MCF-7 breast cancer cells, growth of LCC1 is estrogen independent and anti-estrogen responsive, while LCC9 is both estrogen-independen

SUBMITTER: Soltanian-Zadeh S 

PROVIDER: S-EPMC5976885 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature