Facile isolation of α-ribazole from vitamin B₁₂ hydrolysates using boronate affinity chromatography.

ABSTRACT: Alpha-ribazole (α-R) is a unique riboside found in the nucleotide loop of coenzyme B₁₂ (CoB₁₂). α-R is not an intermediate of the de novo biosynthetic pathway of coenzyme B₁₂, but some bacteria of the phylum Firmicutes have evolved a two-protein system (transporter, kinase) that scavenges α-R from the environment and converts it to the pathway intermediate α-RP. Since α-R is not commercially available, one must either synthesize α-R, or isolate it from hydrolysates of vitamin B₁₂ (cyano-B₁₂, CNB₁₂), so the function of the above-mentioned proteins can be studied. Here we report a facile protocol for the isolation of α-R from CNB₁₂ hydrolysates. CNB₁₂ dissolved in NaOH (5 M) was heated to 85 °C for 75 min

SUBMITTER: Mattes TA 

PROVIDER: S-EPMC5977401 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature