MRNA-seq whole transcriptome profiling of fresh frozen versus archived fixed tissues.

ABSTRACT:

Background

The main bottleneck for genomic studies of tumors is the limited availability of fresh frozen (FF) samples collected from patients, coupled with comprehensive long-term clinical follow-up. This shortage could be alleviated by using existing large archives of routinely obtained and stored Formalin-Fixed Paraffin-Embedded (FFPE) tissues. However, since these samples are partially degraded, their RNA sequencing is technically challenging.

Results

In an effort to establish a reliable and practical procedure, we compared three protocols for RNA sequencing using pairs of FF and FFPE samples, both taken from the same breast tumor. In contrast to previous studies, we compared the expression profiles obtained from the two matched sample types, using the same protocol for b

SUBMITTER: Bossel Ben-Moshe N 

PROVIDER: S-EPMC5977534 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature