Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.

ABSTRACT:

Background

Ever since the first atomic structure of an enzyme was solved, the discovery of the mechanism and dynamics of reactions catalyzed by biomolecules has been the key goal for the understanding of the molecular processes that drive life on earth. Despite a large number of successful methods for trapping reaction intermediates, the direct observation of an ongoing reaction has been possible only in rare and exceptional cases.

Results

Here, we demonstrate a general method for capturing enzyme catalysis "in action" by mix-and-inject serial crystallography (MISC). Specifically, we follow the catalytic reaction of the Mycobacterium tuberculosis β-lactamase with the third-generation antibiotic ceftriaxone by time-resolved serial femtosecond crystallography. The results reve

SUBMITTER: Olmos JL 

PROVIDER: S-EPMC5977757 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature