Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry.

ABSTRACT: Coexpression of proteins in response to pathway-inducing signals is the founding paradigm of gene regulation. However, it remains unexplored whether the relative abundance of co-regulated proteins requires precise tuning. Here, we present large-scale analyses of protein stoichiometry and corresponding regulatory strategies for 21 pathways and 67-224 operons in divergent bacteria separated by 0.6-2 billion years. Using end-enriched RNA-sequencing (Rend-seq) with single-nucleotide resolution, we found that many bacterial gene clusters encoding conserved pathways have undergone massive divergence in transcript abundance and architectures via remodeling of internal promoters and terminators. Remarkably, these evolutionary changes are compensated post-transcriptionally to maintain preferred sto

SUBMITTER: Lalanne JB 

PROVIDER: S-EPMC5978003 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature